What would it take to create and later revive a representative biosphere from frozen stores located on the Moon?
The costs of launchers is getting low enough that we can reasonably imagine the establishment of a lunar base well within NASA’s spaceflight budget.
With the discovery of ices on the lunar poles, astronauts could provide their own life-support indefinitely (water, oxygen, food, and fertilizer). While living in a sheltered habitat, they then immediately proceed to establish other basic processes to step-wise become increasingly independent of supplies from Earth (e.g. producing their own metals and glass).
Given the increasing independence of the small colony, one begins to consider if additional steps could be taken to achieve a fully independent small colony to serve as a backup for the human species should a catastrophe destroy humanity (e.g. a large asteroid or our own self-replicating technology).
We wouldn’t want just for humans to survive, but that other species could eventually be reestablished as well. If species could be stored in their frozen single cell form, millions of individual organisms could be delivered to the Moon in each 5,000 kg payload delivery.
But this leads to some interesting questions:
1) We cannot save all species. There are just too many of them. So, which should we choose in order to have a broad representation of the biosphere?
2) In what biologic form should the frozen specimen be so that they can be most easily revived? Bacteria & protozoa — frozen. Fungi — spores. Plants — seeds. But what about birds, mammals, etc? We can freeze embryos, but how do we get the adult mother to gestate them?
3) How could we eventually establish Minimum Viable Populations? (say 1,000 individuals per species).
It seems to me that these questions could form the basis for interesting biology studies. The more these questions are studied, looking for plausible solutions, the more interest there would be for establishing actual terrestrial and lunar preserves for the biosphere.
Now, if you click on the BioPreserver link on this website, you will learn that the Frozen Ark is doing something rather similar to what is suggested above. However, they focus only on endangered species and not a representation of the whole biosphere. Despite significant affiliations, the rate at which they are securing different species is insufficient to imagine backing up the biosphere in any reasonable number of years.
So please comment on the above ideas and suggest how it could be advanced.