Oxford Nanopore systems offer real-time, scalable, direct DNA sequencing. This can be performed on the portable MinION device, the benchtop GridION and the high-thoughput, high-sample number PromethION.
Nanopore sequencing also offers, for the first time, direct RNA sequencing, as well as PCR or PCR-free cDNA sequencing.
With nanopore sequencing, the user chooses fragment length and the nanopore sequences the entire fragments. Reads approaching 1Mb have been reported.